Imaging single living cells with a scanning near-®eld infrared microscope based on a free electron laser
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چکیده
We report the ®rst sub-wavelength mid-infrared images under water, and describe an application to obtaining images of single living cells in water using the Stanford Free Electron Laser (FEL). Spatial resolution is enhanced at the peak of infrared absorption of water. Images were obtained of single motile ®broblasts with the FEL wavelength tuned to absorption peaks of both protein and lipid molecules. Analysis of the unexpectedly strong absorption due to lipid molecules in motile ®broblasts suggests that the concentration of lipid molecules in lamellopodia is consistent with membrane ̄ow. Ó 1998 Elsevier Science B.V. All rights reserved. PACS: 87.64t; 41.60Cr; 87.64Je; 87.15Mi
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تاریخ انتشار 1998